Journal: bioRxiv

Article Title: Orthogonal CRISPR screens and human liver chimeric mice identify hepatitis B virus host factors

doi: 10.64898/2026.07.23.740386

Figure Lengend Snippet: a Schematic of the generation of human liver chimeric mice with hepatocyte gene KOs followed by HBV infection and FACS-based analysis. b Serial serum hAlb levels of FNRG mice following transplantation of mpPHHs with NT or gene-targeting sgRNAs delivered by lentiviral transduction. Each symbol is the mean; the bars represent the range across mice in each group. Mice per group: n = 6 (NT, IRF2 KO), n = 4 ( ZCCHC14 KO), n = 3 ( WDR48 KO). c Serial serum HBV DNA levels following humanization of FNRG mice are shown in ( a) of NT mice and ZCCHC14 KO mice. The dark line represents the mean for all mice per group; shading represents S.D.; each symbol represents an individual mouse and time point. Mice per group: n = 6 (NT), n = 4 ( ZCCHC14 KO). d Histograms of cell-associated HBsAg signal using FACS. Isolated mpPHHs from a representative isolation. Each histogram represents data from an individual mouse. No Ab, no primary antibody. e Percent of HBcAg-positive isolated mpPHHs measured by FACS. f HBcAg signal of HBcAg-positive isolated mpPHHs measured by FACS. g Serum HBV DNA (x-axis) is plotted against mean HBsAg signal intensity (y-axis). Each symbol represents an individual mouse; all mice from all isolations are included. For d - g , one million isolated mpPHHs from the HBV-infected, NT mouse, including in each isolation, were used for a no-primary-antibody control to establish the HBsAg and HBcAg-negative gates. Data in e and f are presented as mean ± S.D. Each symbol represents an individual mouse. Statistical significance was assessed using two-tailed unpaired t-tests with Welch's correction. * P < 0.05; ** P < 0.01.

Article Snippet: ELISA plates (Thermo Fisher Scientific #4424040) were coated with goat anti-human albumin polyclonal antibody (Bethyl Laboratories #A80–129A), and bound hAlb was detected using HRP-conjugated detection antibody (Bethyl Laboratories #A80–229P).

Techniques: Infection, Transplantation Assay, Transduction, Isolation, Control, Two Tailed Test